SYM-14: Special Topic Symposium: Bioimaging II

DEEP FLUORESCENCE IMAGING IN BIOLOGICAL SAMPLES BY WAVEFRONT SHAPING


Wei Gong1, Ke Si1,2


1Institute of Neuroscience, Zhejiang University, China;
2State Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering Zhejiang University


Fluorescence imaging has revolutionized biomedical research over the past three decades. However one key challenge in all light-based imaging techniques is the fundamentally limited penetration depth of light in biological tissues, as random scattering in biological tissues causes exponential attenuation of the ballistic component of a light wave. Here we present wavefront shaping techniques to address this limitation. One is acoustooptic imaging technique which uses scattering light to achieve high resolution fluorescence imaging up to 2mm deep inside highly scattering media, such as brain slice. The other is deep tissue high resolution technique, in which we use adaptive optics to compensate the wavefront aberration generated by the optical system and the biological sample. These technologies pave the wave for many important applications in both fundamental biology research and clinical studies

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