3D HYDROGELS WITH ELECTRICAL MANIPULATIONS OF PREPOLYMERS AND CELLS
National Taiwan University, Taiwan
3D hydrogel architectures recapitulating biological structures are obtainable by formation and assembly of hydrogel building blocks with reorganized encapsulated cells or particles on an electromicrofluidic platform. This electromicrofluidic platform adopt electrowetting and dielectrophoresis to access varied objects (a) in multiple phases such as prepolymer liquid droplets and crosslinked hydrogels, (b) on a wide range of scales from micrometer cells or particles to millimeter assembled hydrogel architectures, and (c) with diverse properties such as conductive and dielectric prepolymer droplets that are photo, chemically, or thermally crosslinkable. We demonstrate 3D hydrogel architectures, composed of (i) varied particles or cells reorganized in programmable patterns and (ii) biomimetic hydrogels of designed properties and in adjustable geometries, are accessible for the subsequent studeis. We expect the electromicrofluidic platform to be a generic and alternative technique to manipulate cells and hydrogel for reconfigurable 3D bioprinting.