ENHANCED MRNA DELIVERY BY INFLUENZA A VIRUS NON-STRUCTURAL PROTEIN 1 FOR NONVACCINE APPLICATIONS
Yi Liu, Kyle.K.L Phua
National University of Singapore, Singapore
Messenger RNA has shown great potential as an alternative tool for genetic modification of cells. However, transfection efficiency of in-vitro transcribed mRNA is known to be compromised by cell’s innate immune response. Herein we show that non-structural protein 1 (NS1) derived from influenza A virus enhances transfection efficiency of mRNA. By cotransfection with mRNA encoding NS1 protein, higher expression of mRNA encoding green fluorescent protein was achieved in several cell lines compared to controls. Meanwhile, cell viability was unaffected. Enhanced transfection correlated with a reduced interferon response during transfection based on ELISA . In conclusion, we demonstrate that nature inspired antiviral counter schemes such as NS1 is a powerful tool to facilitate the non-vaccine applications of mRNA.